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hcc rapid hybridization solution  (Amersham Life Sciences Inc)
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Amersham Life Sciences Inc hcc rapid hybridization solution
FIG. 1. (A) Northern blot analysis of MMP-9 mRNA expression in liver tissues with <t>HCC.</t> Five micrograms of poly (A)/ RNA from each human tissue was used in the Northern blot analysis. <t>Hybridization</t> was per- formed with human MMP-9 comple- mentary DNA. The 2.8-kilobase band, corresponding to the MMP-9 transcript, was detected. S26r mRNA expression is shown as the corre- sponding internal control in the lower panel. N, nontumorous portion; T, tu- morous portion; and C, portion adja- cent to the capsule. (B) Northern blot analysis of MMP-9 mRNA expression (upper panel) and of corresponding TIMP-1 mRNA expression (middle panel) in liver tissues with HCC. Hy- bridization was performed, as de- scribed in Materials and Methods. Five micrograms of poly (A)/ RNA was loaded into each slot. The 0.9- kilobase TIMP-1 transcript was de- tected in tumorous tissues, in nontu- morous tissues, and in the portions of the tumors adjacent to the capsules. S26r mRNA expression is shown as the corresponding control in the lower panel. N, nontumorous portion; T, tumorous portion; and C, portion adjacent to the capsule.
Hcc Rapid Hybridization Solution, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FIG. 1. (A) Northern blot analysis of MMP-9 mRNA expression in liver tissues with HCC. Five micrograms of poly (A)/ RNA from each human tissue was used in the Northern blot analysis. Hybridization was per- formed with human MMP-9 comple- mentary DNA. The 2.8-kilobase band, corresponding to the MMP-9 transcript, was detected. S26r mRNA expression is shown as the corre- sponding internal control in the lower panel. N, nontumorous portion; T, tu- morous portion; and C, portion adja- cent to the capsule. (B) Northern blot analysis of MMP-9 mRNA expression (upper panel) and of corresponding TIMP-1 mRNA expression (middle panel) in liver tissues with HCC. Hy- bridization was performed, as de- scribed in Materials and Methods. Five micrograms of poly (A)/ RNA was loaded into each slot. The 0.9- kilobase TIMP-1 transcript was de- tected in tumorous tissues, in nontu- morous tissues, and in the portions of the tumors adjacent to the capsules. S26r mRNA expression is shown as the corresponding control in the lower panel. N, nontumorous portion; T, tumorous portion; and C, portion adjacent to the capsule.

Journal: Hepatology

Article Title: Overexpression of matrix metalloproteinase 9 gene in hepatocellular carcinoma with invasive potential

doi: 10.1002/hep.510240206

Figure Lengend Snippet: FIG. 1. (A) Northern blot analysis of MMP-9 mRNA expression in liver tissues with HCC. Five micrograms of poly (A)/ RNA from each human tissue was used in the Northern blot analysis. Hybridization was per- formed with human MMP-9 comple- mentary DNA. The 2.8-kilobase band, corresponding to the MMP-9 transcript, was detected. S26r mRNA expression is shown as the corre- sponding internal control in the lower panel. N, nontumorous portion; T, tu- morous portion; and C, portion adja- cent to the capsule. (B) Northern blot analysis of MMP-9 mRNA expression (upper panel) and of corresponding TIMP-1 mRNA expression (middle panel) in liver tissues with HCC. Hy- bridization was performed, as de- scribed in Materials and Methods. Five micrograms of poly (A)/ RNA was loaded into each slot. The 0.9- kilobase TIMP-1 transcript was de- tected in tumorous tissues, in nontu- morous tissues, and in the portions of the tumors adjacent to the capsules. S26r mRNA expression is shown as the corresponding control in the lower panel. N, nontumorous portion; T, tumorous portion; and C, portion adjacent to the capsule.

Article Snippet: Hybridization was performed at 657C for 2 hours in analysis, tumorous and adjacent nontumorous tissues from 14 HCC Rapid Hybridization Solution (Amersham) with labeled probe.

Techniques: Northern Blot, Expressing, Hybridization, Control, Capsules

FIG. 2. Northern blot analysis of MMP-2 mRNA expression (upper panel) and of corresponding TIMP-2 mRNA expression (middle panel) in liver tissues with HCC. 5 mg of poly (A)/ RNA from each human tissue and 10 mg of total RNA from T98G (a glioblastoma cell line as a positive control) were used in Northern blot analysis. Hybridization was per- formed, as described in Materials and Methods. The 3.1 kilobase MMP-2 transcript and TIMP-2 transcripts of 3.5 and 1.0 kilobase were detected in both tumorous and nontumorous liver tissues from HCC patients. Lower lanes show S26r mRNA ex- pression corresponding to each of the upper lanes. N, nontumorous portion; and T, tumorous portion.

Journal: Hepatology

Article Title: Overexpression of matrix metalloproteinase 9 gene in hepatocellular carcinoma with invasive potential

doi: 10.1002/hep.510240206

Figure Lengend Snippet: FIG. 2. Northern blot analysis of MMP-2 mRNA expression (upper panel) and of corresponding TIMP-2 mRNA expression (middle panel) in liver tissues with HCC. 5 mg of poly (A)/ RNA from each human tissue and 10 mg of total RNA from T98G (a glioblastoma cell line as a positive control) were used in Northern blot analysis. Hybridization was per- formed, as described in Materials and Methods. The 3.1 kilobase MMP-2 transcript and TIMP-2 transcripts of 3.5 and 1.0 kilobase were detected in both tumorous and nontumorous liver tissues from HCC patients. Lower lanes show S26r mRNA ex- pression corresponding to each of the upper lanes. N, nontumorous portion; and T, tumorous portion.

Article Snippet: Hybridization was performed at 657C for 2 hours in analysis, tumorous and adjacent nontumorous tissues from 14 HCC Rapid Hybridization Solution (Amersham) with labeled probe.

Techniques: Northern Blot, Expressing, Positive Control, Hybridization